diff --git a/langchain/text_splitter.py b/langchain/text_splitter.py
index 7c875682..89559505 100644
--- a/langchain/text_splitter.py
+++ b/langchain/text_splitter.py
@@ -439,7 +439,7 @@ class RecursiveCharacterTextSplitter(TextSplitter):
         final_chunks = []
         # Get appropriate separator to use
         separator = separators[-1]
-        new_separators = None
+        new_separators = []
         for i, _s in enumerate(separators):
             if _s == "":
                 separator = _s
@@ -461,7 +461,7 @@ class RecursiveCharacterTextSplitter(TextSplitter):
                     merged_text = self._merge_splits(_good_splits, _separator)
                     final_chunks.extend(merged_text)
                     _good_splits = []
-                if new_separators is None:
+                if not new_separators:
                     final_chunks.append(s)
                 else:
                     other_info = self._split_text(s, new_separators)
diff --git a/tests/unit_tests/test_text_splitter.py b/tests/unit_tests/test_text_splitter.py
index 2da634cd..91730b03 100644
--- a/tests/unit_tests/test_text_splitter.py
+++ b/tests/unit_tests/test_text_splitter.py
@@ -1,4 +1,6 @@
 """Test text splitting functionality."""
+from typing import List
+
 import pytest
 
 from langchain.docstore.document import Document
@@ -148,6 +150,48 @@ def test_metadata_not_shallow() -> None:
     assert docs[1].metadata == {"source": "1"}
 
 
+def test_iterative_text_splitter_keep_separator() -> None:
+    chunk_size = 5
+    output = __test_iterative_text_splitter(chunk_size=chunk_size, keep_separator=True)
+
+    assert output == [
+        "....5",
+        "X..3",
+        "Y...4",
+        "X....5",
+        "Y...",
+    ]
+
+
+def test_iterative_text_splitter_discard_separator() -> None:
+    chunk_size = 5
+    output = __test_iterative_text_splitter(chunk_size=chunk_size, keep_separator=False)
+
+    assert output == [
+        "....5",
+        "..3",
+        "...4",
+        "....5",
+        "...",
+    ]
+
+
+def __test_iterative_text_splitter(chunk_size: int, keep_separator: bool) -> List[str]:
+    chunk_size += 1 if keep_separator else 0
+
+    splitter = RecursiveCharacterTextSplitter(
+        chunk_size=chunk_size,
+        chunk_overlap=0,
+        separators=["X", "Y"],
+        keep_separator=keep_separator,
+    )
+    text = "....5X..3Y...4X....5Y..."
+    output = splitter.split_text(text)
+    for chunk in output:
+        assert len(chunk) <= chunk_size, f"Chunk is larger than {chunk_size}"
+    return output
+
+
 def test_iterative_text_splitter() -> None:
     """Test iterative text splitter."""
     text = """Hi.\n\nI'm Harrison.\n\nHow? Are? You?\nOkay then f f f f.